Class 12 Biology - Biotechnology: Principles and Processes (MCQs) #biologyQuiz #Biotechnology #eduvictors

Class 12 Biology - Principles and Processes (MCQs)

Q1: The linking of antibiotic resistance gene with the plasmid vector became possible with

(a) DNA polymerase 

(b) exonucleases

(c) DNA ligase 

(d) endonucleases

Q2: Which of the following statements does not hold true for restriction enzyme? 

(a) It recognises a palindromic nucleotide sequence

(b) It is isolated from viruses

(c) It is an endonuclease

(d) It can produce the same kind of sticky ends in different DNA molecules

Q3: Autonomously replicating circular extrachromosomal DNA is

(a) plasmid 

(b) capsid

(c) vector 

(d) bacteriophages

Q4: During isolation of DNA, addition of which ofthe following causes precipitation of purified DNA?

(a) Chilled ethanol

(b) Ribonuclease enzyme

(c) DNA polymerase

(d) Proteases

Q5: Agarose extracted from sea weeds finds use in:

(a) Spectrophotometry 

(b) Tissue culture

(c) PCR 

(d) Gel electrophoresis

Q6: The transfer of genetic material from one bacterium to another through the mediation of a viral vector is termed as: 

(a) conjugation

(b) transduction 

(c) transformation 

(d) translation

Q7: The role of DNA ligase in the construction of a recombinant DNA molecule is 

(a) formation of phosphodiester bond between two DNA fragments

(b) formation of hydrogen bonds between sticky ends of DNA fragments

(c) ligation of all purine and pyrimidine bases

(d) None of these

Q8: Polymerase chain reaction is a technique used for

(a) in vivo replication of DNA

(b) in vivo synthesis of mRNA

(c) in vitro synthesis of mRNA

(d) in vitro replication of specific DNA sequence (thermostable DNA polymerase)

Q9: A gene whose expression helps to identify transformed cell is known as

(a) Vector 

(b) Plasmid

(c) Structural gene 

(d) Selectable marker

Q10: Which of the following is not required in the preparation of a recombinant DNA molecule?

(a) Restriction endonuclease 

(b) DNA ligase

(c) E.coli

(d) DNA fragments 

Answers:

1: (c) DNA ligase 

2: (b) It is isolated from viruses

3: (a) plasmid 

4: (a) Chilled ethanol

5: (d) Gel electrophoresis

6: (b) transduction

7: (a) formation of phosphodiester bond between two DNA fragments

8: (d) in vitro replication of specific DNA sequence (thermostable DNA polymerase)

9: (d) Selectable marker

10: (c) E.coli

 

👉See Also:

Biodiversity and Conservation (MCQs)

Biotechnology and Its Applications (Very Short Answer Based Questions - Solved) 

Organisms and Populations (MCQs)

Microbes in Human Welfare (MCQs)

Human Health and Diseases (MCQs)